The Micropropagation of Chestnut Tree: In Vivo Establishment and Post- propagation Growth : Mass Production Technology for Genetically Improved Fast Growing Forest Tree Species

Nangis

Association Foret-Cellulose Paris, IUFRO

1992

S. 421-425

13 Lit. Ang.

Bandaufführung

76081

The objective of the present research is to study in vivo establishment and postpropagation growth of micropropagated plants from several chestnut hybrids clons selected for forest and fruit use. The explants, collected from young stump shoots, were disinfected and multiplied. Shoots from the proliferation system were directly rooted in perlite and composted pine bark mixture in poystyrene trays and then acclimatized under fog-air system. Four weeks after hardening with progressively reduced humidity, the plants were successfully transplanted into different pots with mixes of peat: perlite: composted pine bark or remained in the same polystyrene tray fertilized with a Murashig and Skoog solution, for further growth and development. The last option had better results. In spring the plants were established in nursery for bare root cultivation. Rooting and establishing percentage were close to 100%. One year later the plants had a good ratio between root and aerial portion measured in dry weight. One-year-old plants were used as stockplant for cutting production with a high rooting percentage.