Isolation and characterization of microsatellite markers in the East African tree, Acacia brevispica (Fabaceae: Mimosoideae)

2005

366-368

Sonderdruck

40003278

We have developed primer pairs for 24 microsatellite loci isolated from Acacia brevispica which is widespread in woodland savannas of East Africa. The loci were screened for levels of variation using 16–52 individuals from Mpala Research Centre, Kenya. Number of alleles per locus ranged from two to 17, and polymorphic information content ranged from 0.248 to 0.864. Several loci showed the presence null alleles, but many loci will be useful in ongoing
studies of genetic structure, gene flow, breeding systems, and natural selection.
Keywords: Acacia, gene flow, genetic structure, molecular markers, PCR, SSR