Currently particularly relevant study genetic diversity and intraspecific differentiation of forest trees in connection with the problem of forest genetic resources conservation. Recently, for these purposes are widely used ISSR-markers. The aim of the study is a comparative analysis of ISSR-markers polymorphism and genetic diversity in natural and cultural populations of larch in Perm region. Selected informative ISSR-primers. Analysis of ISSR-spectra plant larch natural population revealed 80 DNA fragments, of which 74 were polymorphic. In trees cultural population found 110 of amplified DNA fragments, of which 96 polymorphic. It is shown that the ISSR-spectra of cultural population characterized by the presence of long DNA fragments in contrast to those of the natural population. The main parameters of the populations genetic diversity are revealed. The proportion of polymorphic loci of the natural population of larch is high and was 0,925, while to those of the cultural population is lower (P95 = 0,872), that is, an cultural population is more genetically homogeneous. Identified a unique ISSR-marker for natural population of larch, which can be used in the preparation of populations molecular genetic formula. Keywords: genetic diversity ; DNA polymorphism ; ISSR-markers ; Larix
