Chapter 5: Sequencing of Complete Chloroplast Genomes

89-105

Artikel aus einem Buch

200208251

In this chapter, frequently used methods for elucidating sequence and structure of chloroplast genomes are reviewed, as a current best practice guide. This concerns methods for DNA extraction, sequencing library preparation, and bioinformatics (assembly, verification, annotation, and sequence comparisons). Recommendations for standard data reporting practices are given—chloroplast genome sequencing reports can be highly formalized, and publication in the form of standard data reports is the best option for comparison and meta-analysis purposes.
Key words: Chloroplast genome High-throughput sequencing Next-generation sequencing Bioinformatics Data reporting standards