Effect of Plant Growth Promoting Rhizobacteria (PGPR) and Cold Stratification on Seed Germination and Early Growth of Corylus avellana L

Wien

2016

S. 337–352

Artikel aus einer Zeitschrift

200201696

Keywords: Pseudomonas putida, hazelnut, seed dormancy, seed vigor index, shoot length
In this study, three putative plant growth promoting rhizobacteria (PGPR) including Pseudomonas putida strain 168, Bacillus subtilis strain FzB24 and Enterobacter cloacae strain 12, were applied as single and combination of three strains for their ability to improve seed germination traits and early growth in three Corylus avellana ecotypes. After inclusion, seeds were sown in 45 cm×20 cm×10 cm plastic containers filled with wet sterilized river sand and subjected to cold stratification (CS) in a refrigerator at (5±1°C) for 0, 3 and 4 months. The experiment was done based on factorial in a completely randomized design with 3 replicates of 20 seeds each. The numbers of germinated seeds were recorded daily for each ecotype. At the end of the experiment, germination percentage (GP), germination speed (GS), seed vigor index (SVI) of the seeds were calculated. Results showed that, CS, ecotypes, bacteria inoculation and interactions of these factors affected germination traits. Over all treatments, GP ranged from 1.66% to 65.40% among the ecotypes. Both CS and inoculation had positive effect on GP. The greatest GP (61.35-65.40%) and onset of germination occurred in seeds co-inoculated by the combination of 3 bacterial inoculants followed by 4 months CS. Among ecotypes, the highest SVI (160.77) belonged to seeds of Makesh ecotype inoculated by the combination of 3 bacterial inoculants full in some points owed by 4 months CS. In case of application of bacterial as individual, the highest germination traits and early growth were obtained in seeds inoculated with Pseudomonas putida. The findings confirm that PGPR is better to be used in combination with cold stratification to overcome seed dormancy, improvement of seed germination treats and early growth of C. avellana.