Analysis of Variation in Chloroplast DNA Sequences.

New York

2014

S. 85-120

Artikel aus einer ZeitschriftUnselbständiges Werk

200187861

This chapter introduces and reviews methods for analyzing variation in chloroplast DNA, mainly by polymerase chain reaction (PCR) and subsequent revelation of polymorphisms. Sources for chloroplast primers are discussed, as well as methods such as Sanger sequencing, PCR followed by restriction fragment length polymorphism (RFLP), gel electrophoresis, fragment analysis on automated DNA sequencers, denaturing high-performance liquid chromatography (dHPLC), and next-generation sequencing (NGS). A special section deals with peculiarities of chloroplast DNA variation, such as tandem repeats and miniand microsatellites.
Chloroplast DNA , PCR , PCR-RFLP , Sanger sequencing , dHPLC , Next-generation sequencing , Gel electrophoresis , Tandem repeats