Maps of the cell wall: using molecular tools to determine polysaccharide arrangement in plant tissues

Hamburg

2007

S. 61-68

2 Tab., 26 Lit. Ang.

Artikel aus einer ZeitschriftUnselbständiges Werk

200151612

The localization of polysaccharides and enzymes in the native cell wall of plant tissues has outlined a great deal about the way the cell wall is laid down, and how it is arranged and modified in growing and senescing tissues. Primary walls are neither homogeneous nor random in their layout, and cell walls in different organs appear to have different physical arrangements to support their different roles. Recent cell wall localization tools that have advanced this understanding of the primary cell wall have ranged from polyclonal antibodies made to multiple epitopes on whole polysaccharides, to monoclonal antibodies specific for polysaccharide fine structure and to antibody-like marker molecules generated from phage display libraries screened for fine structure recognition. They have been used on tissue sections viewed under light and electron microscopy. The use and value of all these tools depends on a very clear understanding of the fine structure of the target polysaccharide, and proof of the structure of the recognition site. The paper reviews the generation of antibody-based cell wall markers and describes their use in outlining the cell wall polysaccharide arrangement and modification in opening, wilted and senesced Sandersonia aurantiaca flower petals.