Identification of Potential Cytogenetical and Biochemical Markers in Bioindication of Common Oak Forests

1996

S. 139-146

27 Lit. Ang.

Unselbständiges Werk

200101885

Cytogenetical and biochemical investigations of common oak (Quercus robur L.) were applied in order to find suitable markers in bioindication of oak populations. Quercus robur karyotype from investigated populations consisted of 24 chromosomes including a satellite pair. One or two small spherical chromatin fragments, "B-chromosomes" were present in some prophase or metaphase cells. Giemsa C-banding, chromomycine A3/DAPI reverse staining and silver impregnation showed the same signal on spherical fragments as on the NORs of satellite chromosomes indicating that "B-chromosomes" were broken NORs. They were entirely heterochromatic, G-C rich, showing AgNO3 positive reaction and they could be used as markers in bioindication of oak populations. Protein bands of about 60 and 95 kDa were distinct in the leaves of heavily damaged trees. Due to its stability isoperoxidases pattern of dormant buds and young leaves might be convenient tissue for biochemical bioindication.