Inoculation of Lodgepole Pine with Four Blue-Stain Fungi Associated with Mountain Pine Beetle, Monitored by a heat Pulse Velocity (HPV) Instrument

1990

S. 31-36

3 Abb., 1 Tab., 23 Lit. Ang.

Unselbständiges Werk

200088529

In two separate experiments in the field, healthy 80-year-old lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm.) were inoculated with four blue-stain fungi that are associated with mountain pine beetle (Dendroctonus ponderosae Hopk.) and beetle-attacked trees. Inocula of Ophiostoma clavigerum (Robins.-Jeff.&Davids.) Harrington, O. huntii (Robins.-Jeff) de Hoog & Scheffer, O. montium (Rumb.) von Arx, and O. minus (Hedgc.) H. & P. Sydow were prepared on sawdust media. Inoculum was placed under the bark in two areas (8x5 cm each), one above the other, separated by a 15-cm space in which heat pulse velocity (HPV) sensors were installed. HPVs in the inoculated trees were monitored daily for 4 weeks. In sections inoculated with O. clavigerum, HPVs were significantly reduced 10 to 13 days after inoculation in both experiments. Sapwood under the inoculated area was visibly altered and colonized by the fungus. The other three fungi did not significantly reduce HPVs. These results proved the capability of O. clavigerum to colonize functional sapwood and stop sap flow within a short period of time and reconfirmed the importance of this fungus in the mortality of trees attacked by mountain pine beetle.