Fluorescence Microscopy. Volume II

1995

457 S.

zahlr. Lit. Ang.

Unselbständiges Werk

0-521-41088-6

200082971

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts. The high sensitivity of the method makes it ideal for studying the distribution of substances in living cells. Its techniques are used mainly in biology and medicine, but are also valuable in coal petrology and elsewhere. The best-known application is in immunofluorescence. This magnificent new work provides comprehensive coverage of all aspects of fluorescence microscopy. It covers instrumentation, applications to a wide variety of fields, and the history of the technique. There is a chapter on quantitative techniques, including scanning: this aspect is dealt with in more detail in a companion volume, Quantitative fluorescence microscopy. Volume II deals with the applications of fluorescence microscopy in many fields. It includes information on fluorochromes and on autofluorescence. An invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.