As part of research into the decline of oak, the mycoflora of healthy and declining Quercus robur individuals was compared on two sites of National Park of Circeo (LT), Central Italy. At each site, two plots of 800 Quadratmeter were dilimited, and the trees located along the diagonals sampled in May (start of the vegetative season) and again in October (start of leaf fall). Fungi isolated were: 1 Ascomycotina and some unidentified Basidiomycontina; 14 identified and 2 unidentified Deuteromycotina. The most common species were Hypoxilon mediterraneum. Diplodia mutila and Phomopsis quercina. An interesting finding in the context of oak decline is that these species were frequently found together.