Organogenesis and Somatic Embryogenesis from the Culture of Walnut (Juglans regia L.)

1990

S. 495-499

1 Abb., 1 Tab.

Unselbständiges Werk

200061603

Axillary shoots were induced with modified DKW medium +6-BA 1.0mg/L from the dormant axillary buds collected from young trees, mature trees and stump sprouts of walnut. The formation of adventitious multiple shoots was observed in the subculture with modified DKW medium +6-BA 1.0mg/L+IBA 0.01mg/L. The efficiency of shoots multiplication was near 600% in one month. After being treated in the medium with IBA 5.0mg/L for 7 days then cultured the medium contained activated carbon, but without growth regulators for 20 days, 54% shoots rooted. Somatic embryos were induced in darkness from immature embryos (from the middle of May to the early of June) cultured in the modified DKW medium +6-BA 1.0 mg/L for 30 days. The somatic embryos germinated in the germinating medium without growth regulators within 7 days in darkness. Suspension culture was established from the embryogenetic callus of walnut.