Biomass analyses were performed in five 134- to 137-year-old sample trees of silver fir to reveal important relations for its vitality and stability. The research was conducted on long-term experimental plots IUFRO/UNESCO at Olomucany near Blansko. Biometric data were estimated for the aboveground and underground parts in volume, fresh and dry weight. Special attention was paid to the share of bark and participation of mistletoe in the total weight of needles and shoots. The architecture of crown and roots was evaluated in the individual components according to crown layers and root depth with differentiation by the quadrants of the cardinal points. The volume of aboveground biomass averages 1,084 Kubikmeter. The average weight represents 1051kg when fresh and 535kg when dry. The root volume attains 0,198 Kubikmeter, fresh weight 183kg and dry weight 97kg. The aboveground and underground biomass totals 1,282 Kubikmeter in volume, 1234kg in fresh weight and 632kg when dry. The results were compared with those obtained previously in a 50-year-old silver fir population of the same origin. The study of fir biomass can be of good use for elucidation of the biological properties and interaction of fir with the environment when searching for most suitable tending methods to prevent its dieback.