Mitotic Index and Influence of Environmental Conditions in the Megagametophyte of Picea abies : 2nd Slovenian Symposium on Plant Physiology with international Participation, Gozd Martuljek. Slovenia, September, 30 to Oktober 2nd, 1998

1999

S. 139-142

2 Abb., 11 Lit.

Unselbständiges Werk

200059339

From May 15 to June 25, 1995 megagametophytes (= female gametophyte, endosperm tissue in development) of spruce trees were collected. 10 preparations of each day were analyzed semi-automatically with regard to their mitotic figures using digital image analysis. All environmental data (temperature, humidity, radiation, atmospheric pressure, rainfall) were measured during this period with a weather station. The data were collected every 5 seconds. From these data averages of a half-hour were calculated. Number of nuclei of one megagametophyte increased from an average of 5000 at the beginning in May to 60.000 at the end of the experiment in June. Correlations were found between temperature, atmospheric pressure and mitotic index. The other parameters showed no connection to the mitotic index. The mitotic index of the megagametophytes were also examined with regard to their location in the cone (top - middle - base). All regions of the cone develop simultaneously - number of nuclei in division showed no significant differences with regard to the position of the cone from which they where collected.