Clonal Propagation of Juvenile and Adult Trees of Sessile Oak by Tissue Culture Techniques

1990

S. 50-55

2 Abb., 4 Tab., 28 Lit. Ang.

Unselbständiges Werk

200051172

Quercus petraea has been propagated in vitro from juvenile and mature explants. Suitable nutrient medium and culture conditions were first determined for explants from 2 to 5 months old seedling; mature explants responded similarly. The best nutrient medium was Gresshoff and Doy's medium supplemented with 0.2 mg/l BA. The nature of the explant (apex, base, node or axillary shoot) had no great influence on multiplication rates; the proliferation coeffiecient was increased by repeatedly culturing mature mother shoots placed horizontally on the medium. Roots formed on shoots placed in half-strength Gresshoff and Doy medium after their bases had been dipped in 0.5 g/l IBA for 6 min or 1 g/l for 2 min. Growth in culture and rooting rates were both markedly affected by the individual plant used as the source of explants.