Micropropagation and Ex Vitro Rooting of Several Clones of Late-flushing Quercus robur L : Genetics of Oaks. IUFRO Proceedings

1993

S. 319-322

11 Lit. Ang.

Unselbständiges Werk

200041675

Green acorns from 11 selected late-flushing Quercus robur trees were used as initial explants for micropropagation. From 60 acorns, 45 clones which produced shoots of suitable quality for ex vitro rooting were obtained. Half- sib clones derived form one mother tree produced an average of 409 microcuttings within 8 months. Half-sib clones of the other 10 trees produced only 11-188 shoots per clone. Microcuttings were rooted ex vitro after treatment with rooting powder containing 0.5% indole-3-butyric acid or 1.0% indole acetic acid. Shoots derived form subcultured shoots tips and nodal segments had a low rooting and survival rate (21%) after 4 months. 56% of shoots derived from subcultured basal segments with a callus, rooted and survived.