Effect of ozone and sulfur dioxide an Norway spruce. Comparative biochemical analysis : Air Pollution and Forest Decline. 14th International Meeting for Specialists in Air Pollution Effects on Forest Ecosystems

1989

S. 131-134

2 Abb., 11 Lit. Ang., En, Zfsg. En

Unselbständiges Werk

200034661

Extracellular biochemical parameters (ascorbate and guaiacol peroxidase, ascorbic aid, and soluble protein) were measured in the intercellular washng fluid (IWF) from the apoplast of current needles of Norway spruce saplings treated with ozone, sulfur dioxide, ambient and filtered air in outdoor fumigation chambers. Saplings were exposed for 24/day during 10-24 weeks to several pollutant concentrations. Exposure to ozone (200 .g/m3) for 12 weeks inhibited ascorbate and guiacol peroxidase activities in the IWF whereas the contents in the same fraction. Exposure to sulfur dioxide (200 .g/m3) for 10 weeks increased both ascorbate peroxidase activity and the contents of ascorbic aid and proteins. The stimulation of these parameters were already statistically significant at a lower concentration of sO2 (70 .g/m3). Values measured in saplings maintained in aient air were comprised between those rom filtered air and pollutant exposed plants. Visible damages were only abserved in saplings exposed to 200 .g 03/m3 for 20-24 weeks. Several of these biochemical parameters were already modified 8 weeks before the appearence of visible damage. The possible use of these biochemical parameters as predictive markers of pollutant injury is discussed.