Gefäßpflanzen ; Genotyp ; Klassifikation ; Methode ; Aufsatzsammlung; Gefäßpflanzen ; Keimplasma ; Genetischer Fingerabdruck ; Aufsatzsammlung; Nutzpflanzen ; Keimplasma ; Genanalyse ; Aufsatzsammlung; Markierungsgen ; RFLP ; Aufsatzsammlung; Gefäßpflanzen ; DNS ; Satelliten-DNS ; Isolierung, Chemie ; Aufsatzsammlung; Nutzpflanzenzüchtung ; Genetischer Fingerabdruck ; Nutzungseignung ; Aufsatzsammlung; DNA fingerprinting of plants ; Pflanzengenetik; Molekularbiologie; The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications once the genotyping data has been generated. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Genotyping: Methods and Protocols is aimed at plant molecular biologists, geneticists, plant breeders and ecologists who have a target question and need to know the most suitable markers and genotyping system to use. Advances in Plant Genotyping: Where the Future Will Take Us; Dhwani A. Patel, Manuel Zander, Jessica Dalton-Morgan, Jacqueline Batley; Molecular Marker Applications in Plants ; Alice C. Hayward, Reece Tollenaere, Jessica Dalton-Morgan; Jacqueline Batley; Bioinformatics: Identification of Markers from Next- Generation Sequence Data; Pradeep Ruperao and David Edwards; Molecular Marker Databases ; Kaitao Lai, Michał Tadeusz Lorenc, and David Edwards; Plant Genotyping Using Fluorescently Tagged Inter-Simple Sequence Repeats (ISSRs): Basic Principles and Methodology ; Linda M. Prince; SSR Genotyping . Annaliese S. Mason; Genotyping Analysis Using an RFLP Assay ; Shutao Dai and Yan Long; DNA Barcoding for Plants ; Natasha de Vere, Tim C. G. Rich, Sarah A. Trinder, Charlotte Long; Multiplexed Digital Gene Expression Analysis for Genetical Genomics in Large Plant Populations . Christian Obermeier, Bertha M. Salazar-Colqui, Viola Spamer, Rod Snowdon 10 SNP Genotyping by Heteroduplex Analysis. Norma Paniego, Corina Fusari, Verónica Lia, and Andrea Puebla; Application of the High-Resolution Melting Technique for Gene Mapping and SNP Detection in Plants ; David Chagné; Challenges of Genotyping Polyploid Species ; Annaliese S. Mason ; Genomic Reduction Assisted Single Nucleotide Polymorphism Discovery Using 454-Pyrosequencing . Peter J. Maughan, Joshua A. Udall, and Eric N. Jellen; Inter-SINE Amplified Polymorphism (ISAP) for Rapid and Robust Plant Genotyping . Torsten Wenke, Kathrin M. Seibt, Thomas Döbel, Katja Muders, and Thomas Schmidt; Screening of Mutations by TILLING in Plants . Nian Wang and Lei Shi; Gene Analysis Using Mass Spectrometric Cleaved Amplified; Polymorphic Sequence (MS-CAPS) with Matrix- Assisted; Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF). Hideyuki Kajiwara; Quantitative SNP Genotyping of Polyploids with MassARRAY and Other Platforms ; Marcelo Mollinari and Oliver Serang; SNP Genotyping Using KASPar Assays . Scott M. Smith and Peter J. Maughan; Skim-Based Genotyping by Sequencing. Agnieszka A. Golicz, Philipp E. Bayer, and David Edwards; The Restriction Enzyme Target Approach to Genotyping by Sequencing (GBS) Elena Hilario; Methods for the Design, Implementation, and Analysis of Illumina Infinium™ SNP Assays in Plants ; David Chagné, Luca Bianco, Cindy Lawley, Diego Micheletti, Jeanne M. E. Jacobs; Use of the Illumina GoldenGate Assay for Single Nucleotide Polymorphism (SNP) Genotyping in Cereal Crops ; Shiaoman Chao and Cindy Lawley
