Genetics of isozyme variants in Populus tremula, P. tremuloides, and their hybrids. : II. Poplar Breeding in the Netherlands

Dordrecht

1991

S. 225-233

Bandaufführung

175964

Eight Populus tremula and six P. tremuloides clones as well as 49 full-sib families were studied in GOT, LAP, 6-PGDH, and SKDH by horizontal starch gel electrophoresis. For GOT one polymophic zone was found and segregation of seven full-sib families suggests Mendelian inheritance. For LAP and 6-PGDH two zones each were clearly scored. For LAP two polymorphic loci were proposed based on the phenotypic segregation of isozyme variants in six and 34 full-sib families, respectively. In 24 full-sibes families the presence of null alleles was inferred for both loci. The genetic control of the upper zone of 6-PGDH was demonstrated by a segregation analysis of 17 full-sib families. SKDH also demonstrated a Mendelian inheritance pattern in 12 of the full-sib families analysed. The electrophoretic patterns of pollen were similar to those of buds, but migration rates of the supposed corresponding isozymes were slightly modified. Lap-A was not present in pollen extracts and hybrid bands were not found when gels were stained for dimeric.
Keywords: Populus tremula, Populus tremuloides, poplar isozymes, genetic control, null alleles, pollen gene expression