Allozyme Markers Detect Mating Compatibility in Non-Clamp-Forming Basidiomycetes : Root and Butt Rots

Uppsala

International Union of Forest Research Organizations, Swedish University of Agricultural Sciences

1994

S. 520-528

12 Lit. Ang.

Bandaufführung

13462

73755

We tested allozyme markers as a method to determine mating compatibility in interbasidiocarp pairings of Phellinus weirii and Phellinus pini. Electrophoretic analysis of singel-spore isolates revealed three segregating loci for Phellinus weirii and one segregating locus for Phellinus pini. After culture plate pairings of all single spores, interaction zone subcultures were again analyzed electrophoretically for the segregating loci. Dikaryotization (indicative of mating compatibility) was shown by the presence of heteroallelism (multiple bands). Results for Phellinus weirii were largely consistent with a bipolar mating system, while Phellinus pini showed a higher incidence of compatibility than expected.