The Origin of Dikaryotic Hyphae in Fruit Bodies of Armillaria Borealis and Armillaria tabescens Developed in Pure Culture : Root and Butt Rots

Uppsala

International Union of Forest Research Organizations, Swedish University of Agricultural Sciences

1994

S. 504-511

10 Lit. Ang.

Bandaufführung

13462

73749

The presence of dikaryotic hyphae in fruit bodies of Armillaria borealis and Armillaria tabescens grown in the laboratory was investigated. Each culture was obtained either by isolating a single uninucleate diploid cell or by taking a mass inoculum from a pairing between two compatible haploid isolates. Three single-cell isolates and three mass isolates of Armillaria borealis as well as one single-cell isolate of Armillaria tabescens fruited successfully. The mass inocula of Armillaria borealis produced fruit bodies with dikaryotic subhymenial tissue, similar to those occurring in nature, whereas the singel- cell isolates produced fruit bodies with diploid subhymenial tissue, different from those in nature. The diploid singel-cell isolate of Armillaria tabescens produced fruit bodies with dikaryotic subhymenium, similar to those in nature. The results obtained with Armillaria borealis suggest that the dikaryotic subhymenial hyphae originate from haploid nuclei preserved within generally diploid mycelium. In Armillaria tabescens an apparent haploidization took place before the development of fruit bodies.