Isolation of 15 polymorphic microsatellite loci in Acacia hybrid ( Acacia mangium × Acacia auriculiformis)

2005

572-575

Sonderdruck

12994S

40003280

This study reports the isolation of 15 microsatellites in Acacia hybrid ( Acacia mangium×Acacia auriculiformis) based on the 5′ anchored polymerase chain reaction technique. Polymorphism of these loci was evaluated in a sample of 24 hybrid individuals. The level of polymorphism ranged from two to eight alleles with an observed heterozygosity ranging from 0.083 to 0.875. These loci were also characterized in both the parental species. The number of alleles ranged from two to six for both A. mangium and A. auriculiformis with the observed heterozygosity values ranging from 0.167 to 0.625 and 0.042 to 0.458 respectively. Five of these loci demonstrated Mendelian inheritance in a segregating F1 population; four presented a distorted ratio and the remaining six did not segregate in progenies as they were homozygous in both parents.Keywords: Acacia auriculiformis, Acacia mangium, Acacia hybrid, microsatellite, tropical tree