Variation in genome size in the Valeriana officinalis complex resulting from multiple chromosomal evolutionary processes

Variabilita ve velikosti genomu Valeriana officinalis jako výsledek mnohočetných evolučních procesů

Prag

2017

S. 41–61

Sonderdruck

12167S

202641

Polyploidy, aneuploidy and change inDNA content of monoploid genomes or chromosomes are the principal causes of the variation in genome size.We studied these phenomena in central-European populations of the Valeriana officinalis complex in order to identify mechanisms or forces driving its evolution. The complex comprises di-, tetra- and octoploid morphologically defined so-called taxonomic “types”. Within the study area there are also intermediate “transitional types” the existence of which hampers the application of traditional taxonomic concepts. We thus chose AFLP genotyping and admixture analyses to identify the genetic structuring of the material studied. Di- (2x), tetra-(4x) and octoploidy (8x) were confirmed as major ploidy levels. Major genetic clusters roughly corresponded to these ploidy levels (for K = 2: 2x- and 8x-clusters, for K = 4 with nearly identical probability: 2x-, 4x-, 8x- and unspecific clusters were identified), which further more significantly differed from each other in monoploid absolute genome size (mean 1Cx for 2x = 1.48 pg, 4x 1.29 pg, 8x 1.10 pg). Several individuals of all ploidy levels were admixed, particularly tetraploids. Relative genome size (the sample: standard DAPI fluorescence) was positively correlated with the proportion of the diploid genetic cluster shared by the tetraploids, indicating that hybridization caused the variation in genome size.K e y w o r d s: aneuploidy, AFLP, chromosome number, evolution, flow cytometry, genome size, hybridization, polyploidy, population, Valeriana