Genetic variation of Norway spruce (Picea abies [L.]Karst.) populations in Austria. II. Microspatial patterns derived from nuclear sequence tagged microsatellite sites

1998

1 Abb., 3 Tab., zahlr. Lit.

Unselbständiges Werk

13343

200055381

In three putatively autochthonous, high elevated Norway spruce populations, microspatial genetic pattern was studied by using three nuclear sequenced tagged microsatellite sites (STMS). In total, 273 trees were genotyped in the Austrian populations. Different measures such as number of alleles per locus (range: 28-38), effective allele number or gene pool diversity Ny (range: 7.6-9.7) (GREGORIUS 1978) and total population differentiation Delta Tau (range:.88-.90) (GREGORUS 1987) indicated a high amount of genetic variation. Expectedly genetic distances among populations were also high. Population differentiation Delta reached .33. Measures were several folds higher than measures based on isozyme data. Spatial patterns of STMS variation were sutdied by spatial autocorrelation using Autocorrelation Indices for DNA Analysis (SIDA) (BERTORELLE & BARBUJANI 1995). Genetic variants were mainly randomly distributed in space. At a certain STMS in one population significant positive II-values in short distance classes and negative II-values in long-distance classes were obtained. This pattern was interpreted as the likely result of selection rather than due to limited gene flow.