Preliminary Investigation of the Use of Indirect Immunofluorescence to Detect Conidia of Brunchorstia pinea

1989

1 Abb., 16 Lit. Ang.

Unselbständiges Werk

9716

200038952

An indirect immunofluorescence staining procedure was developed for detection of Brunchorstia pinea, using antiserum to conidia and a commercially prepared fluorescein isothiocyanate protein A conjugate. The procedure detected B. pinea conida from culture, pycnidia, and sproe-trap collections. Although cross reactivity occurred with spores of Fusarium spp., Sirococcus sp., Phialophora sp., Gliocladium sp., Verticillium sp., and Gelatinosporium sp., these spores were easily distinguished from those of B. pinea by size, shape, septation, and degree of fluorescence. Fluorescent B. pinea like conidia were collected in spore traps located within and outside the New York Stte quarantine region. However, the identity of B. pinea like conidia could not be corroborated by other methods.