Use of p-Nitrophenyl Phosphate for Assay of Soil Phosphatase Activity

Amsterdam

1969

S. 301-307

2 Abb., 4 Tab., 15 Lit. Ang.

Bandaufführung

10217S

145433

A simple method of assaying soil phosphatase activity is described. It involves colorimetric estimation of the p-nitrophenol released by phosphatase activity when soil is incubated with buffered (pH 6 • 5) sodium p-nitrophenyl phosphate solution and toluene at 37° C for 1 hr. The method is rapid and precise, and it has significant advantages over methods previously proposed for assay of soil phosphatase activity.