These proceedings provide a synopsis of the Third Workshop on Genetics of Bark Beetles and Associated Microorganisms held May 20 and 21, 2006, in Asheville, NC. The first workshop was held at the University of California, Berkeley on May 17 and 18, 1992. It was organized by Jayne Hayes and Jacqueline Robertson to address the increasing realization that our understanding of bark beetle biology and phylogeny suffered from a fundamental lack of knowledge about their genetics. Further, research on bark beetles had made relatively minor use of recent and emerging technologies in addressing this gap. There were 23 participants. The workshop had a very informal structure, without assigned speaking orders, which promoted vibrant discussion. The final session was devoted to an open discussion of critical needs for future research. The proceedings are reported in USDA Forest Service, General Technical Report PSW-GTR-138, 31 p.