Polish Sudeten and Carpathian Mountains Silver-fir (Abies alba) Population Genetic Investigations : 9th International European Silver Fir Symposium : 9. Internationales IUFRO-Tannen-Symposium
Single-tree silver-fir (Abies alba) megagametophyte isozymes were analyzed with starch-gel electrophoresis. Trees were collected from 9 Carpathian and 2 Sudeten populations. 21 to 29 isozyme loci were analyzed. Additonally one remnant population the Sniezka (Snow Mount) from Sudeten were analyzed. Very significant genetic distances between the Sudeten and Carpathian Mountains populations were revealed. The lowest expected and observed (He and Ho respectively) herzozygosity was observed in Sudeten populations: He = 0.110 and Ho = 0.120. The mean expected and observed heterozygosity amounted to 0.240 and 0.260 respectively and was similar compared to that in the other conifers. Maximum heterozygosity amounted to He = 0.290 and Ho = 0.390. The mean number of alleles per locus (Na) varied between 1.16 in Miedzygorze in Sudeten Mts to 2.43 in Lesko (Carpathian Mts.). The Mean effective number of alleles per locus (Ne = 1.40) ranged from 1.19 in Sudeten Ladek Zdroj population to 1.65 in Carpathian Lesko population. Wright's fixation index Fis revealed in 7 populations excess of heterozygotes and in 4 populations excess of homozygotes. In the remnant population average heterozygosity amounted Ho = 1.160 and for polymorphic loci Hopoli = 0.274. Average number of allele / locus equaled Na = 1.87 and effective number of allele/locus Ne = 1.33. Above quoted results show us that even so small population like Sniezka still retain significant amount of genetic diversity.
