2-D separation of Verticillium albo-atrum proteins

Laibach

University of Ljubljana

2006

S. 455-460

1 Abb., 22 Lit. Ang.

Artikel aus einer ZeitschriftUnselbständiges Werk

200135648

Acta agriculturae Slovenica 2006, 87(2), S. 455-460

Verticillium albo-atrum is a phytopathogenic fungus that causes wilt disease in a wide range of crops. A proteomic approach is needed to obtain a comprehensive overview of the proteins related to the infection process. We report here on the optimisation of electrophoretic analysis of total proteins of V. albo-atrum in terms of the extraction of fungus total proteins, removal of interfering Compounds and Separation of proteins by two-dimensional electrophoresis.