Conservation of Valuable Gene Resources by Cryopreservation in some Forest Tree Species

1990

S. 373-376

3 Abb., 4 Tab., 8 Lit. Ang.

Unselbständiges Werk

200050476

Journal of Plant Physiology 1990 : Biochemistry, Physiology and Molecular Biology of Plants ; 136(3), S. 373-376

It is demonstrated that pollen, small seeds and immature embryos can be deep- frozent at -196C without loss of viability. The survival rate of the tested pollen and seeds (with the exception of oak) was 100%of controls. In the case of embryos with high humidity the survival rate was higher tha 90% when using a special cryoprotectant and a special freezing technique. These results are especially important for species with large seeds with high moisture content, such as oak, beech and horse-chestnut, where long-term storage is not possible without rapid loss of germination capacity.