Eingewanderte und eingeschleppte Schadorganismen

Wien

Bundesamt und Forschungszentrum für Wald

2004

S. 6-10

4 Abb.

Artikel aus einer ZeitschriftUnselbständiges Werk

200114558

Forstschutz Aktuell 2004, (31) : Forstschutzsituation 2003 in Österreich : Erhebungen und Diagnosen des BFW und Dokumentation der Waldschädigungsfaktoren 2003, S. 6-10

Among the most dangerous imported products are stones and electronic equipment from PR China shipped in containers with solid wood packaging material. Due to strong phytosanitary import requirements the number of detected living stages of organism (e.g. wood boring insects, sapwood-nematodes) decreased compared to the years before. The exact diagnosis of imported quarantine longhorn beetles in each ontogenetic stage is of special interest to the Plant Protection Service. Morphological features give less evidence for reliable determination done by non-specialists. Also the morphological determination of early stages like egg and larvae is nearly impossible. Molecular investigations were initiated in Austria with the aim to develop a non-morphological diagnostic method for determination of Anoplophora glabripennis and its differentiation from other Anoplophora species, from imported and native Monochamus species, and other native longhorned beetles. DNA from adults, larvae, pupae and eggs was amplified with mitochondrial primers and PCR (polymerase chain reaction) products were digested with restriction enzymes (PCR-RFLP (restriction fragment length polymorphism). Based on the mitochondrial PCR-RFLP method it is possible to cleary distinguish A. glabripennis from the other four Anoplophora species and also from the three Saperda- and ten Monochamus-species involved and to differentiate one from the other by digesting one or two PCR fragment(s) with five (or eight) different restriction enzymes providing species specific RFLP patterns.