On the genetic variation of Abies alba Mill. monitored by isozyme markers as revealed from the provenance trial "Knödelhütte 1967" : 9th International European Silver Fir Symposium : 9. Internationales IUFRO-Tannen-Symposium

2002

S. 55-62

16 Lit. Ang.

Unselbständiges Werk

200076113

Proceedings of the 9th International European Silver Fir Symposium May 21-26,2000 in Skopje - Republic of Macedonia : 9th International European Silver Fir Symposium : 9. Internationales IUFRO-Tannen-Symposium ; 08, S. 55-62

Abies alba Mill. represents an indispensable ecological and functional element of mixed mountain forests. Reduced adaptability as a consequence of insufficient genetic variability has been considered a major cuase for silver fir decline in central and northeastern Europe. Testing this hypothesis, several provenance trials and investigations were started during the 80's. All the samples investigated in this study were collected from provenances, which are growing in a provenance trial carried out at research nursery and aboretum "Knödelhütte" from the University of Agricultural Sciences, Vienna (Austria) between 1967 and 1970. The goal of this study was to find out, whether the provenances trial "Knödelhütte 1967" can be proofed to be representative by means of isozyme gene markers and to assess to which extend this provenance trials is already biased by age. Seventeen gene loci were electrophoretically analysed by dormant buds collected from 282 trees belonging to 15 Silver fir provenances and one provenance of Abies nordmanniana (Stev.) Sprach. On the average, all the silver fir provenances had a proportion of polymorphic loci (P95) of 48.60%; the number of alleles per locus (Al) was 1.77; the actual heterozygosity (HA) reached 0.13; the total population differentiation of the gene pool exhibited a 0.17 value; the gene pool diversity (Vpool) howed a result of 1.20; and the hypothetical gametic diversity (Vgam) was 42.62. The subpopulation differentiation Dj was used for demonstrating geographic variation and genetic drift in some provenance plots. With some few exceptions, all the data of allelic structure and the genetic parameters matched well the known data from literature. This was interpreted as an optimal representativity of the selected provenances for their genetic regions. In view of these results, isoenzyme analysis is recommended as tool for planning, when the representativity of selected provenances and collected samples has to be tested.